Autores UPV
Glaied Ghram Imen,
Belguith Hatem,
Messaoudi Abdelmonaem,
Fattouch Sami,
Vicente Meana Óscar,
Ben Hamida Jeannette
Abstract
Lipases are useful enzymes that are responsible for the hydrolysis of triacylglycerides and play an
important role in plant growth. In this study, we report a rapid molecular method to amplify a partial
sequence of the lipase class 3 family designated BnLIP3 gene of Brassica napus L. in order to follow its
expression and analyze its role during seed germination. Therefore, we conceived PCR homologous
primers to amplify the active site encoding region of the BnLIP3 family genes. Subsequently, to
sequence determination of the 582 bp fragment, we deduced BnLIP3 specific primers for a nested RTPCR
application. The deduced 194 amino acid sequence (Genbank 1160264) was found to share 85% of
identity with lipase from Arabidopsis thaliana class 3 family. The GxSxG consensus motif near the
catalytic triad at the active serine site was also identified. The peptidic sequence showed little
homology with mammalian and microbial lipases. RT-PCR analysis indicated that BnLIP3 gene was
expressed during B. napus seed germination.
