Thin-Layer Chromatographic Image Analysis for the Determination of Sulfide Ions Using Pyrylium Cations

Autores UPV
Revista JPC - Journal of Planar Chromatography - Modern TLC


A highly specific and sensitive chromatographic method for the determination of sulfide ions was developed. The procedure is based on the sensitive and specific reaction of sulfide ions with 4-[p-(N,Ndimethylamino) phenyl]-2,6-diphenylpyrylium cations. The product of the reaction is a thiopyrylium derivative which can be quantitatively determined. Separations were performed on cellulose thinlayer chromatography (TLC) plates with a mixture of phosphoric buffer (pH 3.0), propan-2-ol, and acetonitrile (4:4:0.5, v/v) as the mobile phase. The thiopyrylium derivative was visible in the form of blue spots against a colorless background, which were converted into a chromatogram using TLSee software. Linearity was obtained in the range of 40¿500 pmol per spot. The limits of detection and quantification were 10 pmol per spot and 33 pmol per spot, respectively. The proposed method was successfully used for the detection and determination of sulfide ions in water samples from the Busko Zdrój health resort.