Abstract
The detection of pathogenic bacteria is key for the
identification and prevention and of problems related to health
and safety. Accurate and quick detection and quantification of
pathogen microorganisms in food, drinking water and in clinical
diagnostics using cost-effective methodologies are greatest
challenges currently facing food industry, health and
environmental fields. A rapid and sensitive biosensor, based on
immunological methods, was developed for bacteria detection
using LightScribe disc as platform. We are able to detect between
0 and 106
CFU/mL in only 1.5 hours without the need for sample
pre-concentration. The cross-reactivity study and individual
ready-to-eat meat samples were inoculated with live bacteria to
demonstrate proof-of-concept for real samples.