Improvement of antigen detection using protein A for the oriented bio-functionalization of integrated photonic biosensor

Autores UPV
Año
CONGRESO Improvement of antigen detection using protein A for the oriented bio-functionalization of integrated photonic biosensor

Abstract

The objective of this work is to optimize the detection of antigens with integrated photonic sensing structures by using a protein A layer to promote bio-functionalization with specific antibody probes. Protein A allows the oriented attachment of antibody receptors over the sensor surface, since they bind to the protein layer through their Fc section and their Fab sections will be oriented towards the sample to be analyzed, thus enhancing the interaction with the target antigens to be detected. The attention was focused on a well-known antigen typically used as model in experimental development: bovine serum albumin (BSA).