Autores UPV
Molina Botella María Inmaculada,
Martinez-Sanchis Juan Vicente,
Pertusa Grau Jose Francisco,
Balasch Parisi Sebastià,
Iniesta Ignacio,
Pellicer Antonio
Abstract
Objective: To demonstrate the usefulness of image analysis in designing objective embryonic morphometric variables.
Design: Retrospective study of 214 top-quality day-2 embryo photographs from 50 double-embryo transfers resulting in no pregnancy
(group 0) and 57 resulting in twin pregnancy (group 1).
Setting: Human reproduction unit.
Patient(s): Study of 107 in vitro fertilizationintracytoplasmic sperm injection (IVFICSI) cycles in women age<36 years with doubleembryo
transfer of top-quality embryos. Only the first cycle of IVFICSI was included.
Intervention(s): Standard IVFICSI protocols.
Main Outcome Measure(s): The embryo photographs were analyzed using the ImageJ program. The effects of the embryo variables
and the clinical variables on embryo implantation were evaluated using a stepwise dichotomous logistic regression.
Result(s): Significant differences were observed, owing to the women's ages, internal perimeter, roundness factor, and zona pellucida
thickness. Embryos with smaller internal perimeter, circular shape, and thinner zona pellucida were more likely to implant.
Conclusion(s): Morphometric variables lower the subjectivity of the current embryo grading systems. These variables are nonsubjective
factors to consider when predicting implantation. Embryo image analysis is an accurate tool
that can improve IVFICSI outcomes and reduce the number of twin pregnancies. (Fertil Steril
2014;102:10228. 2014 by American Society for Reproductive Medicine.)
Key Words: Embryo selection, embryo score, morphological and morphometric embryo
variables, images analysis, embryo implantation, embryo grading systems