Fishing for Phytophthora 2.0

Autores UPV
Año
CONGRESO Fishing for Phytophthora 2.0

Abstract

Isolation of Phytophthora species from water sources is common and worldwide known. Usually, the most common methods used are baiting in situ and filtering of water. Both methods are successful for the isolation of aquatic species included in clade 6 like P. gonapodyides, P. megasperma, P. taxon PgChlamydo or P. lacustris. However, only a few species from other clades are isolated using these methods due to the relative low inoculum available. Therefore, a study was performed to compare the number of Phytophthora species isolated using baits and the number of Phytophthora species detected using pyrosequencing technology. A total of 16 forest streams from northern Spain were selected for the study. Water samples were filtered in situ and the filters were used for isolation and for pyrosequencing studies. Additionally, baits (rhododendron leaves and carnations petals) were placed in the same streams and collected after one week. Isolation from baits were performed using selective media and amplicon pyrosequencing was performed from each sample. Results compared data among culturing of Phytophthora species from filters and baits, and those species detected by pyrosequencing.