Discovering Phytophthora species in the laurel forest in Tenerife and La Gomera islands (Canary Islands, Spain)

Autores UPV
Año
CONGRESO Discovering Phytophthora species in the laurel forest in Tenerife and La Gomera islands (Canary Islands, Spain)

Abstract

A survey in the laurel forest was performed in three different sites in Tenerife (Anaga Rural Park, Corona Forestal Park and Teno Rural Park) and in La Gomera (Garajonay National Park) during January 2013. Water and soil samples were collected and DNA was extracted using E.Z.N.A. and Zymo DNA kits respectively. Amplicon library generation was performed using a nested PCR with Phytophthora-specific primers. A total of 100.095 sequences were obtained from a single pyrosequencing run and used to assess Phytophthora species diversity. In total, 24 Phytophthora species were detected, 22 species (P. gonapodyides, P. megasperma, P. taxon PgChlamydo, P. gregata, P. taxon oaksoil, P. lacustris, P. taxon walnut, P. asparagi, P. cryptogea, P. drechsleri, P. syringae, P. plurivora, P. multivora, P. quercetorum, P. cactorum and Phytophthora sp1, sp2, sp3, sp4, sp5, sp6 and sp7) were found in Tenerife and 11 species (P. gonapodyides, P. megasperma, P. taxon PgChlamydo, P. taxon oaksoil, P. hydropathica, P. europea, P. quercetorum and Phytophthora sp2, sp5, sp6 and sp7) were detected in La Gomera. Seven of these Phytophthora species are new to science. Phytophthora taxon walnut was detected in a water sample from Tenerife (Anaga Rural Park), with a total of 65 sequences, which could represent a new world location of this species. Fifteen of the species identified by pyrosequencing are also commonly detected in mainland Spain. This study showed that Macaronesian Islands could be considered as a hotspot for genus Phytophthora research due to the isolation and species evolution with their hosts.