Living biointerfaces based on non-pathogenic bacteria to direct cell function

Autores UPV
CONGRESO Living biointerfaces based on non-pathogenic bacteria to direct cell function


Understanding cell behaviour on synthetic surfaces is of foremost interest to engineer microenvironments that direct cell fate. As cells cannot interact directly with synthetic biomaterials, surfaces have been functionalized with a broad range of proteins, fragments, peptides and growth factors. However, these passive coatings can by no means provide the dynamic stimuli required to orchestrate cell responses and organise the formation of a new tissue at the material interface. Significant efforts have focused on engineering materials that recapitulate characteristics of ECM, such as the presentation of cell adhesive motifs or protease degradable cross-links, in order to direct cellular responses1. However, the development of a cell/material interface able to provide biological stimuli upon demand has not been established yet. We hypothesised that non-pathogenic bacteria can provide such a role, as they can colonise the surface of a broad range of materials and can be genetically modified to express or secrete desired adhesive proteins or factors to a living cell population, upon demand. This work investigates the potential of a living interface based on Lactococcus Lactis expressing a FN fragment (FNIII7- 10) as a membrane protein to direct cell adhesion, signalling and differentiation2. This system introduces engineered bacteria as a new tool to direct cell behaviour at the material interface.