Molecular evidence for ongoing complementarity and horizontal gene transfer in endosymbiotic systems of mealybugs

Autores UPV
Año
Revista Frontiers in Microbiology

Abstract

Intracellularbacterialsupplyofessentialaminoacidsiscommonamongsap-feedinginsects,thuscomplementingthescarcityofnitrogenouscompoundsinplantphloem.Thisisalsotheroleofthetwomealybugendosymbioticsystemswhosegenomeshavebeensequenced.InthenestedendosymbioticsystemfromPlanococcuscitri(Pseudococcinae),¿CandidatusTremblayaprinceps¿and¿CandidatusMoranellaendobia¿cooperatetosynthesizeessentialaminoacids,whileinPhenacoccusavenae(Phenacoccinae)thisfunctionisperformedbyitssingleendosymbiont¿CandidatusTremblayaphenacola.¿However,littleisknownregardingtheevolutionofessentialaminoacidsupplementationstrategiesinothermealybugsystems.Toaddressthisknowledgegap,wescreenedforthepresenceofsixselectedlociinvolvedinessentialaminoacidbiosynthesisinfiveadditionalmealybugspecies.WefoundevidenceofongoingcomplementarityamongendosymbiontsfrominsectsofsubfamilyPseudococcinae,aswellashorizontalgenetransferaffectingendosymbiontsfrominsectsoffamilyPhenacoccinae,providingamorecomprehensivepictureoftheevolutionaryhistoryoftheseendosymbioticsystems.Additionally,wereporttwodiagnosticmotifstohelpidentifyinvasivemealybugspecies.