Toward a more efficient isolation of total RNA from loquat (Eriobotrya japonica Lindl.) tissues

Autores UPV
Revista Fruits


Introduction. Loquat (Eriobotrya japonica Lindl.) tissues contain considerable amounts of polyphenolic compounds and polysaccharides which can copurify with RNA. This can render the RNA unsuitable for DNA synthesis and makes the isolation of high quality RNA from loquat tissues particularly difficult. Materials and methods. Total RNA was extracted from leaves, buds and fruit of loquat by adapting the method described by Song et al. in 2011. This involved improvements both to the extraction protocol and RNA purity. Results and discussion. Our modified method resulted in the isolation of high quality RNA with yields 6-20 times higher than that reported for existing methods. We obtained A(260/280) ratios of 2-2.40 A(260/230) ratios of 2-2.31 and RNA integrity numbers (RIN) of 8 to 8.3. The RNA obtained has reached the requested quality for downstream applications such as quantitative real-time polymerase chain reaction (RT-qPCR) experiments.