A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard

Autores UPV
Revista Plant Methods


Conclusions: The functionality and the efficiency of gRNA-Cas9 GB tools were demonstrated in Nicotiana benthamiana using transient expression assays both for gene targeted mutations and for transcriptional regulation. The availability of gRNA-Cas9 GB toolbox will facilitate the application of CRISPR/Cas9 technology to plant genome engineering.