Lichen microalgae biotechnology: Novel approaches to efficiently isolate and culture the hidden diversity of microalgae in a single thallus

Autores UPV
Año
CONGRESO Lichen microalgae biotechnology: Novel approaches to efficiently isolate and culture the hidden diversity of microalgae in a single thallus

Abstract

Lichens are usually described as mutualistic and cyclical symbioses between a fungus and photosynthetic microorganisms, termed phycobionts (green microalgae or/and cyanobacteria). At present, lichen thalli are considered to be an example of micro--‐ ecosystems due to the coexistence of several phycobionts inside a single thallus, as well as the proven presence of lichen--‐associated bacteria whose functional contexts are supposed to play a beneficial role for the symbiotic sustainability of thalli. Most lichen studies were based only on the SANGER methodology that probably underestimated the potential microalgal heterogeneity. In 2010, our ¿Lichen Symbiogenesis Team¿ developed a simple and rapid method to isolate lichen phycobionts, which permits the isolation of many pure, easily identifiable, axenic colonies of microalgae. Isolated microalgae of lichen species included in this study have been characterized with molecular and ultrastructural techniques. Our results suggest that this method may be an appropriate protocol in order to more efficiently reveal the hidden green--‐microalgae diversity inside a single thallus, and to obtain pure axenic colonies of these symbiotic algae for physiological analysis as well as technological purposes in order to study their industrial viability. The possible use of lichen microalgae symbionts for high value compounds and for other uses in the biotechnological industry requires the development of studies which can screen the bioprocess conditions in order to demonstrate the bioactivity of the compounds and their technological aptitude, which will allow the development of viable processes in the industry. The aim of this study was to obtain a faster and more accurate methodology to fully analyse the potential and unknown diversity of the symbiotic primary producers so this can be used in biotechnological industries. (MINECO, CGL2012--‐40058--‐C02--‐01), FEDER, (GVA, PROMETEOII/2013/021).