Autores UPV
Fuente Lorente Lorena de la,
Arzalluz Luque Ángeles,
Tardáguila Manuel,
Hector del Risco,
Martí Ibáñez María Cristina,
Tarazona Campos Sonia,
Salguero García Pedro,
Raymond Scott,
Alberto Lerma,
Alastrue-Agudo A,
Pablo Bonilla,
Jeremy R.B. Newman,
Shunichi Kosugi,
Lauren M. McIntyre,
Moreno-Manzano Victoria,
A. Conesa
Abstract
Recent advances in long-read sequencing solve inaccuracies in alternative transcript identification of full-length transcripts in short-read RNA-Seq data, which encourages the development of methods for isoform-centered functional analysis. Here, we present tappAS, the first framework to enable a comprehensive Functional Iso-Transcriptomics (FIT) analysis, which is effective at revealing the functional impact of context-specific post-transcriptional regulation. tappAS uses isoform-resolved annotation of coding and non-coding functional domains, motifs, and sites, in combination with novel analysis methods to interrogate different aspects of the functional readout of transcript variants and isoform regulation. tappAS software and documentation are available at https://app.tappas.org.
